A Modified Precipitation Method to Isolate Urinary Exosomes
نویسندگان
چکیده
منابع مشابه
A modified precipitation method to isolate urinary exosomes.
Identification of biomarkers that allow early detection of kidney diseases in urine and plasma has been an area of active interest for several years. Urinary exosome vesicles, 40-100 nm in size, are released into the urine under normal conditions by cells from all nephron segments and may contain protein, mRNA and microRNA representative of their cell type of origin. Under conditions of renal d...
متن کاملUrinary Exosomes
Exosomes are nanovesicles of endocytic origin that are secreted into the extracellular space or body fluids when a multivesicular body (MVB) fuses with the cell membrane. Interest in exosomes intensified after their description in antigen-presenting cells and the observation that they can significantly moderate immune responses in vivo. In the past few years, several groups have reported on the...
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Abstract Background and objectives: Ureaplasma urealyticum is one of the most important causes of Nongonococcal urethritis (NGU) and non-specific urethritis (NSU) in men. Also Mycoplasma hominis has a role in causing NGU and NSU. The aim of this study was the detection of Mycoplasma hominis & Ureaplasma urealyticum in men suffering from NGU and NSU by prostatic massage method and first- void Ur...
متن کاملUrinary exosomes: is there a future?
Tiny vesicles called ‘exosomes’, recently discovered in normal urine [1], provide a non-invasive means of acquiring unique information about the physiological or pathophysiological state of their renal cells of origin. Exosomes are delivered to the urine from all renal epithelial cell types. Consequently, analysis of urinary exosomes may provide a source of protein biomarkers for diseases invol...
متن کاملHuman urinary exosomes as innate immune effectors.
Exosomes are small extracellular vesicles, approximately 50 nm in diameter, derived from the endocytic pathway and released by a variety of cell types. Recent data indicate a spectrum of exosomal functions, including RNA transfer, antigen presentation, modulation of apoptosis, and shedding of obsolete protein. Exosomes derived from all nephron segments are also present in human urine, where the...
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ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2015
ISSN: 1940-087X
DOI: 10.3791/51158